Mucosal tissue model formed in vitro experimental cell biology. Part 1. Method for constructing the mucosal tissue model and its histological observation.:Part 1, method for constructing the mucosal tissue model and its histological observation

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The mucosal tissue model (M. M.) was constructed in vitro by culturing the cells in collagen lattice. M. M. consists of the submucosal tissue model (S. M. M.) and epidermal cells which grew out from the epithelial fragment on S. M. M. In fabricating S. M. M., fibroblasts derived from rabbit oral mucosa were brought into the conditioned collagen solution, and were cultured in three-dimentional condition for 7 days. Fibroblasts were trapped in collagen lattice, and the original size of collagen lattice was gradually reduced. Thus, S.M. M. was fabricated. Subsequently, the mucosal epithelial fragment, which was taken from oral mucosa of the same rabbit, was put onto S. M. M. The fragment on S. M. M. was cultured under the same conditions. Cultivated for 7 days, the epithelial cells from the fragment covered the surface of S. M. M. as the confluent cell sheet. Cultivated for 28 days, a regular basal cell layer was found with overlying strata of differentiating cells. The epidermis became thick, and the incipient pegs penetrating S. M. M. were seen. The fabricating M. M. proved to be useful for investigating mucosal wound healing and mucosal disease in vitro, and for mucosal tissue graft.
社団法人日本口腔外科学会の論文