Daikenchuto, a Kampo Medicine, Regulates Intestinal Fibrosis Associated with Decreasing Expression of Heat Shock Protein 47 and Collagen Content in a Rat Colitis Model
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概要
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Heat shock protein (HSP) 47 may play an important role in the pathogenesis of intestinal fibrosis. Daikenchuto (DKT), a traditional Japanese herbal (Kampo) medicine, has been reported to ameliorate intestinal inflammation. The aims of this study were to determine time–course profiles of several parameters of fibrosis in a rat model, to confirm the HSP47-expressing cells in the colon, and finally to evaluate DKTs effects on intestinal fibrosis. Colitis was induced in male Wistar rats weighing 200 g using an enema of trinitrobenzene sulfonic acid (TNBS). HSP47 localization was determined by immunohistochemistry. Colonic inflammation and fibrosis were assessed by macroscopic, histological, morphometric, and immunohistochemical analyses. Colonic mRNA expression of transforming growth factor β1 (TGF-β1), HSP47, and collagen type I were assessed by real time-polymerase chain reaction (PCR). DKT was administered orally once a day from 8 to 14 d after TNBS administration. The colon was removed on the 15th day. HSP47 immunoreactivity was coexpressed with α-smooth muscle actin-positive cells located in the subepithelial space. Intracolonic administration of TNBS resulted in grossly visible ulcers. Colonic inflammation persisted for 6 weeks, and fibrosis persisted for 4 weeks after cessation of TNBS treatment. The expression levels of mRNA and proteins for TGF-β1, HSP47, and collagen I were elevated in colonic mucosa treated with TNBS. These fibrosis markers indicated that DKT treatment significantly inhibited TNBS-induced fibrosis. These findings suggest that DKT reduces intestinal fibrosis associated with decreasing expression of HSP47 and collagen content in the intestine.
著者
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Yoshikawa Toshikazu
Department Of Endocrinology And Metabolism Graduate School Of Medical Science Kyoto Prefectural Univ
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KONISHI HIDEYUKI
Department of Gastroenterology Kyoto Prefectural Yosano-umi Hospital
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HIRATA Ikuhiro
Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
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Wakabayashi Naoki
Department Of Internal Medicine Kyoto Prefectural Yosanoumi Hospital
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Naito Yuji
市立奈良病院 消化器肝臓病センター
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Naito Yuji
Department Of Cardiovascular Surgery Ntt East Corporation Sapporo Hospital
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Fukumoto Kohei
Department Of Gastroenterology And Hepatology Nara City Hospital
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Yamada Shinya
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Takagi Tomohisa
Department Of Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine
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Yoshida Naohisa
Department Of Gastroenterology And Hepatology Nara City Hospital
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Hayashi Natsuko
Department Of Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine
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Inoue Ken
Department Of Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine
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Takagi Tomohisa
Department Of Molecular Gastroenterology And Hepatology Graduate School Of Medical Science Kyoto Pre
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Handa Osamu
Department Of Molecular Gastroenterology And Hepatology Graduate School Of Medical Science Kyoto Pre
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Kokura Satoshi
Department Of Molecular Gastroenterology And Hepatology Graduate School Of Medical Science Kyoto Pre
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Uchiyama Kazuhiko
Department Of Molecular Gastroenterology And Hepatology Graduate School Of Medical Science Kyoto Pre
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Mizushima Katsura
Department Of Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine
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Ichikawa Hiroshi
Department Of Electrical And Electronic Engineering Shizuoka University
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Omatsu Tatsushi
Department Of Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine
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Ishikawa Takeshi
Department Of Chemistry And Materials Engineering Faculty Of Chemistry Materials And Bioengineering
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Inoue Ken
Department Of Electronic Engineering Denki-tsushin University (uec)
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Harusato Akihito
Department Of Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine
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Yagi Nobuaki
Department Of Molecular Gastroenterology And Hepatology Graduate School Of Medical Science Kyoto Pre
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Hirai Yasuko
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto P
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Horie Ryusuke
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto P
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Naito Yuji
Department Of Cardiovascular Center Hokkaido Social Insurance Hospital
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Handa Osamu
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Konishi Hideyuki
Department of Chemistry, Aichi Kyoiku University
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YOSHIKAWA TOSHIKAZU
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Konishi Hideyuki
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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YAGI Nobuaki
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Yamada Shinya
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Naito Yuji
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Harusato Akihito
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Kokura Satoshi
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Hayashi Natsuko
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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WAKABAYASHI Naoki
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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UCHIYAMA KAZUHIKO
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Hirai Yasuko
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Hirata Ikuhiro
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Ishikawa Takeshi
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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TAKAGI TOMOHISA
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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Wakabayashi Naoki
Department of Gastroenterology and Hepatology, Otsu Municipal Hospital, Japan
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Omatsu Tatsushi
Department of Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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ISHIKAWA TAKESHI
Department of Chemical Engineering, Faculty of Engineering, Tokyo Metropolitan University
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