A METHOD TO ACCURATELY INJECT TUMOR CELLS INTO THE CAUDATE/PUTAMEN NUCLEI OF THE MOUSE BRAIN

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概要

• 論文の詳細を見る

• OBJECTIVE: To improve currently used techniques to implant tumor cells into the parenchyma of the mouse brain. MATERIALS AND METHODS: The stereotactic injection of 0.5 to 5 μl of indigo carmine over 5 to 40 minutes into the caudate/putamen nuclei of the mouse was done followed by sacrifice and examination of the brain injection site. 1 μl containing 10^5 U87MG glioma cells were stereotactically implanted into the caudate/putamen nuclei over 20 minutes. The animals were sacrificed from one hour to 63 days after implantation and the brain examined and tumor size measured. RESULTS: An injection of 1 μl of indigo carmine over 20 minutes produced a spherical deposit of dye within the caudate/putamen nuclei. Larger volumes of indigo carmine or shorter injection times resulted in dye spreading along the injection tract or into the ventricles or subarachnoid space. Using the results of the dye studies, the same parameters were used to successfully inject and confine the glioma cells to the caudate/putamen nuclei in 30 of 32 mice. No tumor was found in 2 animals and appears to be explained by obstruction of the injection cannula. The tumor cells appeared viable an hour after injection. However by day three, considerable necrosis of tumor cells were noted, the effects of which resolved by day five. On day six, the injection site was comparable to that at one hour. In the early phase, until the fifth week, tumor volume doubling time was ten days while afterward it was only five days. CONCLUSION: The technique described allows the highly accurate and reproducible introduction of a given number of cells into a specific area of the mouse brain. This should reduce the intragroup variability, be it control or therapeutic, allowing better assessment of outcome with fewer number of mice.

• 東海大学の論文

著者

• YAMADA Shinya

  Department of Physics, The University of Tokyo

• Suzuki Atsushi

  Hematology-oncology Childrens Hospital Los Angeles And The Keck School Of Medicine University Of Sou

• Yamada Shinya

  Department Of Neurosurgery Tokai University Oiso Hospital

• Yamada Shinya

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Yamada Shinya

  東海大学医学部附属大磯病院 外科学系

• KHANKALDYYAN Vazgen

  Hematology-Oncology, Childrens Hospital Los Angeles and The Keck School of Medicine, University of S

• BU Xiang

  Hematology-Oncology, Childrens Hospital Los Angeles and The Keck School of Medicine, University of S

• GONZALEZ-GOMEZ Ignacio

  Department of Pathology, Childrens Hospital Los Angeles and The Keck School of Medicine, University

• TAKAHASHI Kouichi

  Divisions of Neurosurgery, Childrens Hospital Los Angeles and The Keck School of Medicine, Universit

• McCOMB J.

  Divisions of Neurosurgery, Childrens Hospital Los Angeles and The Keck School of Medicine, Universit

• LAUG Walter

  Hematology-Oncology, Childrens Hospital Los Angeles and The Keck School of Medicine, University of S

• Mccomb J.

  Division Of Neurosurgery Children's Hospital Los Angeles And The Keck School Of Medicine Univer

• Laug Walter

  Hematology-oncology Childrens Hospital Los Angeles And The Keck School Of Medicine University Of Sou

• Bu Xiang

  Hematology-oncology Childrens Hospital Los Angeles And The Keck School Of Medicine University Of Sou

• Khankaldyyan Vazgen

  Hematology-oncology Childrens Hospital Los Angeles And The Keck School Of Medicine University Of Sou

• Gonzalez-gomez Ignacio

  Department Of Pathology Childrens Hospital Los Angeles And The Keck School Of Medicine University Of

• Takahashi Kouichi

  Divisions Of Neurosurgery Childrens Hospital Los Angeles And The Keck School Of Medicine University

• McCOMB J.

  Divisions of Neurosurgery, Childrens Hospital Los Angeles and The Keck School of Medicine, University of Southern California

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