各種培養条件下におけるAspergillus oryzaeの菌体内cyclic AMPおよびcyclic GMPレベルについて

概要

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Intracellular adenosine 3', 5'-cyclic monophosphate (cAMP) and guanosine 3', 5'-cyclic monophosphate (cGMP) levels of Aspergillus oryzae grown under various conditions were measured. Levels of cAMP in cells grown on wheat-bran or a 50:50 mixture of defatted soybean and crushed wheat were 6 to 20-fold higher than those grown in glucose-peptone medium. Levels of cAMP in cells grown on the solid medium containing high moisture were lower than those grown on the solid medium containing low moisture. In both the solid and the liquid cultures cellular cAMP level declined during the early growth phase and increased during the late growth phase. On the other hand, cGMP decreased to minimal levels at the end of the growth phase under various cultural conditions. In the range of 20°C to 40°C, cAMP level and cAMP/cGMP ratio increased with increasing temperature. Intracellular cAMP and cGMP levels of A. oryzae were concluded to depend greatly upon the cultural condition.
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