Aspergillus niverの5'-ホスホジエステラーゼ高生産変異株の造成

概要

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A strain of black koji-mold, which hyperproduced 5'-phosphodiesterase, was isolated from soil. This strain was identified as Aspergillus niger by its taxonomical characteristics.An 8-azaadenine and 5-fluorocytosine resistant mutant FS-44 was successfully obtained from the above strain by treatments with ultraviolet light and N-methyl-N'-nitro-N-nitrosoguanidine. The resistant strain showed 5.5 times higher activity of 5'-phosphodiesterase than the parent strain, though it resembled the parent strain in appearance.Phosphomonoesterase in the resistant strain was inactivated by heat treatment at 80℃, pH 4.8 for 12 to 24 sec in 0.5% sodium laurylsulfate solution. 5'-phosphodiesterase solution from which phosphomonoesterase activity had been eliminated by this treatment was applied to an RNA digest prepared by nuclease P_1digestion. The 5'-phosphodiesterase treatment dimminished the dinucleotide fraction and gave 4% increase of the 5'-mononucleotide fraction.
社団法人日本生物工学会の論文
1979-07-25