酵母のクエン酸発酵機構に関する予備試験(酵母の有機酸発酵に関する研究-9-)

概要

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Aconitase and NAD-or NADP-linked isocitric dehydrogenases in yeasts and Aspergillus niger were examined in relation to citrate fermentation. In the cases of Asp. niger, the activities of each enzyme decreased obviously at the stage of citrate accumulation. In the cases of yeasts, however, those activities did not decrease so much throughout the period of citrate fermentation. In a ferrous ion-restricted medium, Candida lipolytica could accumulate a larger amount of citrate, accompanied by a reduced amount of d-isocitrate, as seen before. It was proved that, in this case, the restricted concentration of ferrous ion caused the decrease of the activity of aconitase. In a thiamine-restricted medium, C. lipolytica could accumulate a large amount of α-ketoglutarate, accompanied by reduced amounts of citrates, as seen before. In connection with this, it was proved that restriction in the concentration of thiamine intensified the activities of isocitric dehydrogenases.
社団法人日本農芸化学会の論文