Candida lipolytica によるクエン酸発酵の機構について(酵母の有機酸発酵に関する研究-10-)

スポンサーリンク

概要

論文の詳細を見る
The mechanism of citrate fermentation, especially the anaplerotic pathways of oxalacetate, was studied by use of resting cells of Candida lipolytica. In the early stage of incubation, glucose consumption by resting cells was slightly higher when glucose was employed as substrate. At the same time, polyol production was observed even in the presence of neutralizing agent, but citrate production proceeded at a low level. After a short time, polyol production ceased and citrate production increased. The pretreatment of resting cells with starvation culture medium was extremely detrimental to citrate production by the cells. These observations were discussed in connection with the postulated mechanism of inhibition of isocitrate dehydrogenase by internal factors of the cells. Resting cells grown in glucose medium began to produce citrates even from acetate or n-paraffins in the presence of surface-active agents after a short time lag. It was simultaneously revealed that isocitrate lyase and malate synthase, the key enzymes of the glyoxylate cycle, were formed inducibly. Fluoroacetate, at a concentration of 10 mg per ml, stimulated citrate production extremely when glucose was employed as substrate, but depressed it slightly when n-paraffins were employed. These results suggest that oxalacetate, which is drained off in citrate accumulation, may be supplied through three-carbon precursor (s) plus carbon dioxide fixation when glucose was employed as substrate, and through the glyoxylate cycle when acetate or n-paraffins were employed. It seemed that citrate fermentation by yeasts, except that by fluoroacetate-sensitive mutant strains of C. lipolytica, (6) could be characterized by relatively high levels of the specific activity of aconitate hydratase.
社団法人日本農芸化学会の論文

著者

関連論文

もっと見る

スポンサーリンク