乳児糞便のBifidus因子活性およびL.bifidus var.pennsylvanicusによる糞便糖質の利用 (Lactobacillus bifidusの代謝に関する研究-1,2-)

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On the modified Tomarelli's medium, L. bifidus var. pennsylvanicus ATCC 11863 showed an increase in growth with supplements of human milk or α, β-ethyl-N-acetyl-D-glucosaminide. The bifidus factor activity of human milk was markedly higher than that of cow's milk. Faeces of a breast-fed infant had, in comparison with that of a bottle-fed infant, the bifidus factor activity of a definitely much higher order and the ratio of activity was about 40 : 1. The activity corresponded to the amounts of amino sugar in the faeces. The strain showed distinct growth on medium prepared from faeces of a breast-fed infant, indicating that the medium contained sufficient amounts of the bifidus factor and energy source for the strain. The large amounts of oligo- and polysaccharides in the medium were utilized by the strain. The strain failed to grow on media prepared from faeces of bottle-fed and mixed-fed infants by ten-fold dilution unless they were supplemented with lactose and the bifidus factor. However, good growth was obtained when the media were prepared from them by five-fold dilution and supplemented with lactose. This indicates that almost sufficient amounts of the bifidus factor were being supplied from the faeces.
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