Effect of Cortisol <I>in vivo</I> on Amino Acid Incorporation into Protein by Rat Liver Ribosomes <I>in vitro</I>
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概要
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Effect of cortisol administration in vivo on the activity of protein synthesis in ribosomal and polysomal preparations from rat liver was examined. Purified ribosomes obtained from adrenalectomized and cortisol-treated rats showed a higher activity of amino acid incorporation in the presence of poly U in vitro than those from adrenalectomized, untreated rats. Higher activity of poly U-directed amino acid incorporation in ribosomes from the hormone-treated animals was retained even after elimination of endogenous m-RNA by preincubation, and therefore, it was concluded that the activity of ribosomes was altered by cortisol-treatment.<BR>Significant increase in heavy polysomes was observed 4 hr after administration of the hormone. Purified polysomes were fractionated on sucrose gradient, and the analysis of the fractions for amino acid incorporating activity revealed that light polysomes obtained from cortisol-treated rats were twice as active as those from control animals, while no significant difference was detected in the activity of heavy polysomal fractions.
- 社団法人 日本内分泌学会の論文
著者
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ICHII SHOGO
Division of Physiology, Institute of Steroid Research and lst Department of Internal Medicine
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OMATA SABURO
Division of Physiology, Institute of Steroid Research, Tottori University School of Medicine
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OMATA SABURO
Division of Physiology and Pathology, National Institue of Radiological Sciences
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ICHII SHOGO
Division of Physiology and Pathology, National Institue of Radiological Sciences
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