Interaction of <SUP>3</SUP>H-Dexamethasone-Receptor Complex with Nuclei: Inhibition by Ethidium Bromide
スポンサーリンク
概要
- 論文の詳細を見る
Several binding characteristics of the Dexamethasone (DEX)-receptor complex (RC) to nuclei from rat tissues were examined in the presence of Ethidium Bromide (EB). In the cell-free experiments, EB partially prevented the binding of the liver DEX-RC to nuclei and two kinds of nuclear binding of the DEX-RC, one was sensitive and the other was resistant to EB, were observed. In addition, the EB-resistant nuclear binding that showed a high-affinity and low-capacity nature was observed only in the bindings of the<SUP>3</SUP>H-DEX-RC to nuclei derived from the liver of fetal and adult rats and the thymus of adult rats. The bindings of<SUP>3</SUP>H-5α-Dihydrotestosterone (DHT)-RC and<SUP>3</SUP>H-17β-Estradiol (E<SUB>2</SUB>)-RC to liver nuclei exhibited low-affinity and non-saturation even in the presence of EB. Furthermore, it was revealed that the EB-resistant sites of the nuclear binding were for the “DNA-unbound” form of<SUP>3</SUP>H-DEX-RC, while the RC of “DNA-bound” form was bound to the EB-sensitive sites.
- 社団法人 日本内分泌学会の論文
著者
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ICHII SHOGO
Division of Physiology, Institute of Steroid Research and lst Department of Internal Medicine
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IZAWA MASAO
Division of Physiology, Institute of Steroid Research, Tottori University School of Medicine
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IZAWA MASAO
Division of Physiology, Institute of Steroid Research, Tottori University School
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ICHII SHOGO
Division of Physiology and Pathology, National Institue of Radiological Sciences
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