ニジマス卵抽出液中のリン含有ペプチドの性状

概要

論文の詳細を見る
Phosphopeptides containing large quantities of serine and phosphorus were found in the trichloroacetic acid extract of matured eggs of rainbow trout, Salmo gairdneri, and could be precipitated from the extract with ethanol. When the ethanol concentration of the extract was increased, the amount of precipitate was increased; the peptides obtained at 90% ethanol concentration amounted to about 1% of the rainbow trout eggs and contained more than half of both the nitrogen and the phosphorus of the extract. These peptides emerged in the position of the X-component on the cation exchange column chromatography. Their amino acid composition resembled that of the X-component in large amounts of serine, aspartic acid, arginine, alanine, etc., and in less amounts of aromatic amino acids and sulfur-containing amino acids. However, the peptides seemed to be a mixture of the same kind of phospho-peptides, judging from the facts that the nitrogen/phosphorus molar ratios of the precipitates obtained at various ethanol concentrations were not the same, that the peptides obtained at 90% ethanol were separated into two fractions by dialysis, and that some peaks were observed in an electrophoretic diagram of the higher molecular fraction.
公益社団法人日本水産学会の論文