STUDIES ON MUSCLE OF AQUATIC ANIMALS-XXXII:THE INTRINSIC VISCOSITY OF FISH-ACTOMYOSIN

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The intrinsic viscosity of actomyosin fraction purified by repeating the dissolution with a moderate concentration of NaCl solution (0.6M NaCl+M/30 Na2HPO4+M/30 KH2PO4, pH6.8) and the precipitation by dilution with water was determined. The fraction was proved by the salting-out analysis to be consisted of a practically single component precipitatable between 28-34% of saturated ammonium sulphate (Fig. 1). The values of intrinsic viscosity for eight species of fish came within a narrow range, 2.0-2.5, with the exception of a shotted halibut, having the lower value (1.8) (Fig. 2). When the fishes were frozen and stored, those values were lowered with the time of storage, and the lowering rate was found to differ from species to species (Fig. 3).
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