Purification and characterization of poly(ADP-ribose) polymerase from Sarcophaga peregrina
スポンサーリンク
概要
- 論文の詳細を見る
Poly(ADP-ribose) polymerase (PARP) was purified from embryonic NIH-Sape-4 cells of Sarcophaga peregrina almost to homogeneity. The purification was carried out using phosphocellulose and 3-aminobenzamide-Sepharose column chromatographies. The purified enzyme had an apparent molecular weight of 113kDa on SDS-polyacrylamide gel electrophoresis. The addition of DNA was essential for the activity of the purified enzyme, and the reaction was stimulated by the presence of histone and magnesium ion. The Km for NAD was 43μM and the optimum temperature for the PARP activity ranged from 20-25°C. MALDI (matrix-associated laser desorption ionisation)-TOF (time-of-flight) mass spectrometric analysis gave a signal at 112, 402. This value was close to 113, 033, the predicted mass from its cDNA sequence. This suggests that full length PARP is present as a major native form without extensive post-translational modification.
- 日本学士院の論文
著者
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SUGIMURA Takashi
Biochemistry Division, National Cancer Center Research Institute
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NOZAKI Tadashige
Biochemistry Division, National Cancer Center Research Institute
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Nozaki T
National Cancer Center Res. Inst. Tokyo Jpn
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Natori Shunji
Faculty Of Pharmaceutical Science The University Of Tokyo
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Masutani Mitsuko
Biochemistry Division National Cancer Center Research Institute
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ESUMI Hiroyasu
Biochemistry Division, National Cancer Center Research Institute
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IKEJIMA Miyoko
Biochemistry Division, National Cancer Center Research Institute
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KURATA Shoichiro
Faculty of Pharmaceutical Sciences, University of Tokyo
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Noda T
National Cancer Center Hospital Tokyo
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Kurata S
Univ. Tokyo Tokyo Jpn
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Kurata Shoichiro
Faculty Of Pharmaceutical Sciences The University Of Tokyo
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Sugimura Takashi
Biochemistry Division National Cancer Center
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Kurata Shoichiro
Faculty Of Pharmaceutical Science The University Of Tokyo
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Natori Shunji
Facult Of Parmaceutical Sciences The University Of Tokyo
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Esumi Hiroyasu
Biochemistry Division National Cancer Center Research Institute
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