Assay for lipid peroxide content in mitochondria by the thiobarbituric acid reaction.

スポンサーリンク

概要

• 論文の詳細を見る

• The thiobarbituric acid (TBA) reaction method established by Yagi to determine the lipid peroxide level in serum and animal tissues was applied to isolated mitochondria with some modifications. The TBA reaction was carried out in acetic acid (pH 2.0) in the presence of 0.7 % sodium dodecyl sulfate at 95°C for 45 min. Chloroform was used to remove the lipid and protein from the reaction mixture. An aqueous layer was used for spectrophotometric measurement at 532.5 nm to determine the TBA reaction product with lipid peroxide. To evaluate the TBA assay system, the lipid peroxide content of mitochondrial suspensions exposed to ultraviolet light was examined. The lipid peroxide formed in mitochondria was proportional to the dose of ultraviolet energy. The lipid peroxide of methyl linoleate exposed to ultraviolet light was determined both with the TBA reaction and with the hydroperoxide reaction. The linear increase in TBA values following UV exposure was similar to the increase of hydroperoxide values determined iodometrically. The TBA assay was shown to be a good predictor of lipid peroxide formed in mitochondria. This assay system is useful for lipid peroxide determination in various tissues: epidermis, Ehrlich ascites tumor cells and B-16 melanoma culture cells.

• 久留米大学医学部 The Kurume Medical Journal 編集部の論文

著者

• Nagata Osamu

  Department Of Anesthesiology Tokyo Women's Medical University

• OGURA RYOHEI

  Department of Biochemistry, Kurume University School of Medicine

• SUGIYAMA Masayasu

  Department of Medical Biochemistry, Kurume University School of Medicine

• KAJIYAMA KIMINORI

  Department of Medical Biochemistry, Kurume University School of Medicine

• HIDAKA TOSHIHIRO

  Department o f Medical Biochemistry, Kurume University School of Medicine

• SAKANASHI TOSHIHIKO

  Department of Medical Biochemistry, Kurume University School of Medicine

• NAKAGAWA TETSURO

  Department of Medical Biochemistry, Kurume University School of Medicine

• SHIN GENKO

  Department of Medical Biochemistry, Kurume University School of Medicine

関連論文

• A comparison of the absolute amplitude of motor evoked potentials among groups of patients with various concentrations of nitrous oxide
• Self-expandable metallic stent placement for patients with inoperable esophageal carcinoma : investigation of the influence of prior radiotherapy and chemotherapy
• Odorant evoked magnetic fields in humans
• Epidural anesthesia for cesarean delivery in a parturient with a double-outlet right ventricle
• Body movement during propofol anesthesia appears to be related to the individual rather than to environmental conditions
• Effect of monochromatic ultraviolet light on lipoperoxide formation and on the function of isolated rat liver mitochondria.
• Effect of anti-inflammatory agents on the release of acid phosphatase in the epidermis following ultraviolet light exposure.
• Alterations in Beta-Adrenergic Receptor Density and Cyclic-AMP Level in the Myocardium of Rats Chronically Treated with Alcohol.
• Subcellular distribution of acid DNase of rat submandibular gland.
• Assay for flavins in rat heart mitochondria by high pressure liquid chromatography.
• Arachidonate Metabolism in Bovine Gallbladder Mucosa.
• Electron spin resonance studies on the spin label of rat cardiac mitochondria treated with adriamycin.
• Cloning and Expression of cDNA for Soluble Form of Rat Heme Oxygenase-l.
• UV Exposure alters the membrane lipid composition and cell membrane fluidity of intact cultured B-16 melanoma cells.
• Effects of ultraviolet irradiation on cultured B-16 melanoma cells - Membrane fluidity, lipid composition and phospholipase activity.:Membrane Fluidity, Lipid Composition and Phospholipase Activity
• DEVIL'S DISEASE IN AN UNUSUALLY YOUNG BOY:THE INCIDENCE, CONSIDERATION ON ETIOLOGY AND THERAPEUTIC TRIAL WITH CORTICOSTEROID
• Riboflavin Deficiency Caused by Treatment with Adriamycin.
• THE OXYGEN UPTAKE CAUSED BY THE CYSTEINE UTILIZATION OF RAT EPIDERMIS
• Identification of Free and Esterified Hydroxyoctadecadienoates (HODEs) and Linoleate-Hydroxylating Pathway in the Epidermis of Hairless Mice.
• Biochemical and morphological changes in isolated rat liver mitochondria exposed to ultraviolet light.
• ESR Spin-labeling method of determining membrane fluidity in biological materials. Tissue culture cells, cardiac mtiochondria, erythrocytes and epidermal cells.:Tissue Culture Cells, Cardiac Mitochondria, Erythrocytes and Epidermal Cells
• THE POSSIBLE ROLE OF GLUTATHIONE TO LACTATE DEHYDROGENASE ACTIVITY IN THE EPIDERMIS
• Inhibitory action of water-extract of cow snout epidermis on mushroom tyrosinase.
• Ultrasonic and X-ray tomographic findings of Schistosomiasis Japonicum.
• BIOCHEMICAL AND SCANNING ELECTRON MICROSCOPIC PROPERTIES OF MITOCHONDRIA ISOLATED FROM RAT CARDIAC MUSCLE
• Study of acid DNase in cow snout epidermis by micro slab electrophoresis using acidic and basic gel sheet.
• THE FUNDAMENTAL STUDY ON THE MECHANISM OF THE CALCULI FORMATION (I)
• Assay for lipid peroxide content in mitochondria by the thiobarbituric acid reaction.
• STUDIES ON THE DEPOSITION OF CALCIUM SALT
• BIOCHEMICAL CHANGES IN RAT EPIDERMIS INDUCED BY CHRONIC ULTRAVIOLET-LIGHT EXPOSURE
• Biochemical role of glutathione on the sulfhydryl disulfide interchange reaction with randomly cross-linked ribonuclease.
• INFLUENCE OF HAIR GROWTH CYCLE ON SULFHYDRYL GROUPS OF RAT EPIDERMIS
• A new simplified radioisotope method for determining hypoxanthine-guanine phosphoribosyltransferase and adenine phosphoribosyltransferase with the use of thin-layer radio-chromatogram scanner.
• Disorders Ca2+ homeostasis in rat cardiac mitochondria affected by adriamycin.

もっと見る 閉じる

スポンサーリンク