ESR Spin-labeling method of determining membrane fluidity in biological materials. Tissue culture cells, cardiac mtiochondria, erythrocytes and epidermal cells.:Tissue Culture Cells, Cardiac Mitochondria, Erythrocytes and Epidermal Cells
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概要
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The spin-labeling technique with electron spin resonance (ESR) was introduced to determine membrane fluidity in plasma membranes. Three kinds of doxylstearic acid were used as spin labeling agents. The experimental conditions of ESR measurement, such as spin probe concentration, number of cells, decay of ESR specturum, incubation time, ESR cavity temperature, and microwave power, were discussed with the aim of obtaining reproducible ESR spectra. As biological material, B-16 melanoma cells, Ehrlich ascites tumor cells, epidermal cells, erythrocytes and cardiac mitochondria were used.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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OGURA RYOHEI
Department of Biochemistry, Kurume University School of Medicine
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SUGIYAMA Masayasu
Department of Medical Biochemistry, Kurume University School of Medicine
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SAKANASHI TOSHIHIKO
Department of Medical Biochemistry, Kurume University School of Medicine
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NINOMIYA TSUYOSHI
Department of Medical Biochemistry, Kurume University School of Medicine
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