Electron spin resonance studies on the spin label of rat cardiac mitochondria treated with adriamycin.
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概要
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Adriamycin (ADR), which is a potent antitumor agent, has severe cardiotoxicity; however, the underlying mechanism is not well understood. In our present study, the electron spin resonance (ESR) technique was employed to analyze the physical properties of cardiac mitochondrial membrane treated with ADR. After intercalcation of spin probe into the isolated cardiac mitochondrial membrane, mitochondria were incubated with 3.45×10<SUP>-4</SUP>M ADR for 10 minutes at 37°C, the membrane fluidity was measured by ESR spin probe; 5-doxyl stearic acid (5-DSA) and. 12-doxyl stearic acid (12-DSA). The ADR in vitro treatment resulted in an elevation of the order parameter S and S (T_??_). It was suggested that the membrane fluidity of cardiac mitochondria decreased in both 5-DSA and 12-DSA regions of the lipid bilayer.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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Iwahashi Hiroshi
Department Of Ophthalmology Osaka Police Hospital
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OGURA RYOHEI
Department of Biochemistry, Kurume University School of Medicine
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SUGIYAMA Masayasu
Department of Medical Biochemistry, Kurume University School of Medicine
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KAJIYAMA KIMINORI
Department of Medical Biochemistry, Kurume University School of Medicine
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HIDAKA TOSHIHIRO
Department of Medical Biochemistry, Kurume University School of Medicine
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HIDAKA TOSHIHIRO
Department o f Medical Biochemistry, Kurume University School of Medicine
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ENTMAN MARK
Section of Cardiovascular Sciences, Department of Medicine, The Methodist Hospital and Baylor College of Medicine
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SHIMADA IKUKO
Department of Medical Biochemistry, Kurume University School of Medicine
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IWAHASHI HIROSHI
Department of Medical Biochemistry, Kurume University School of Medicine
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