UV Exposure alters the membrane lipid composition and cell membrane fluidity of intact cultured B-16 melanoma cells.

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The response of eukaryocytes such as cultured B-16 melanoma cells to oxidative stress has been examined following ultraviolet (UV) exposure. Changes of lipid peroxide content, membrane lipid composition and cell membrane fluidity were measured. Lipid peroxide content, expressed as malondial-dehyde content, increased immediately after UV exposure and then rapidly decreased to less than the non-exposed controls, 6 hrs after UV exposure. The polyunsaturated fatty acid (PUFA) content, especially the content of arach-idonic acids (AA), of the phospholipids of the cells increased and the chole-sterol/phospholipid molar ratio decreased, 6 hrs after UV exposure. No further change was observed in phospholipid class distribution of the cells. These re-sults indicate that eukaryocytes, such as B-16 melanoma cells, have the ability to repair or remodel damaged membranes, partly through replacement of oxidized fatty acids in membrane phospholipids via deacylation and reacylation. On the other hand, the cell membrane fluidity, measured by spin label techniques, decreased immediately after UV exposure and then increased 6 hrs after UV exposure. It can be concluded that the biphasic change of cell membrane fluidity reflects the repair or remodeling of the membrane damaged by oxidation.
久留米大学医学部 The Kurume Medical Journal 編集部の論文