A new simplified radioisotope method for determining hypoxanthine-guanine phosphoribosyltransferase and adenine phosphoribosyltransferase with the use of thin-layer radio-chromatogram scanner.
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概要
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An electrophoretic method for determining hyppoxanthine-guanine and adenine phosphoribosyltransferase in a mammalian tissue is presented. The method based on Kizaki's micro-assay procedure was improved with modification using a technic of thin-layer radio-chromatographic scanning. The modification serves to reduce the time in the enzyme assay without any interference among nucleotide, nucleoside and other decomposition product. In this paper, the assay was applied to crude extract of cow snout epidermis, and the salvage enzyme activity in the epidermis was determined.
- 久留米大学医学部 The Kurume Medical Journal 編集部の論文
著者
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Kumano Shuji
Department Of Dermatology Kurume University School Of Medicine
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OGURA RYOHEI
Department of Biochemistry, Kurume University School of Medicine
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Koga Hideaki
Department of Gastroenterology and Hepatology, Saiseikai Nakatsu Hospital, Japan
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KOGA HIDEAKI
Department of Medical Biochemistry, Kurume University School of Medicine
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KUMANO SHUJI
Department of Medical Biochemistry, Kurume University School of Medicine
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