Oxidation of 14C-labeled glucose and palmitic acid in brain microvessels isolated from rats with acute hepatic failure.

概要

論文の詳細を見る
A study was conducted to investigate oxidation of 14C-labeled glucose and palmitic acid in brain microvessels (BMV) isolated from rats with D-galactosamine induced hepatic injury (D-Ga1N) or acute ischemic hepatic failure (AIHF). Following results were obtained: 1) BMV isolated from D-Ga1N as well as AIHF showed significantly lower oxidation of D-(U-14C)-glucose as compared with control. 2) BMV isolated from AIHF also showed significantly lower oxidation of (1-14C)-palmitic acid as compared with control. 3) In BMV isolated from AIHF, uptake of 2-deoxy D-(1-14C)-glucose was rather enhanced as compared with control. 4) Sera obtained from AIHF, in paticular a fraction with molecular weight higher than 10, 000, inhibited in vitro the oxidation of D-(U-14C)-glucose in BMV isolated from normal rats. In conclusion, reduction of energy metabolism in BMV is clearly demonstrated in rats with hepatic failure, and is further suggested to be presumably due to circulating toxins. Additional studies will be required to elucidate mechanism(s) that regulate a relationship between reduced energy metabolism in BMV and development of brain edema in acute hepatic failure.