Characterization of the receptor for polymerized human serum albumin on hepatitis B virus

概要

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The existence of the receptor for polymerized human serum albumin (pHSA) on hepatitis B virus (HBV-pAR) is reported for attachment of HBV to human hepatocytes. However, the characterization of the HBV-pAR is still unclear. In the present study, the characterization of HBV-pAR was investigated by SDS-PAGE, Western-blotting using monoclonal antibody to HBV-pAR (TS-1), HRPO-pHSA and HRPO-anti-HBs, and high performance liquid chromatography (HPLC) methods. The HBV-pAR preparated from purified HB surface antigen (HBsAg) particles was consisted of 5 kind of paptide 67, 52, 35, 31 and 8 K daltons (Kds), and the determinant of HBsAg was consisted of 6 kinds of peptide 67, 52, 35, 31, 27 and 22 Kds. On the other hand, the main component of the peptide 67 Kd was human serum albumin (HuSA) and it was indicated by the use of HPLC Mono-Q prepacked HR 5/5 column that the HBV-pAR activity was contained in the other 3 to 4 components differentiated from HuSA. These results was supported that the peptide 8 Kd is contained in each peptide with HBV-pAR, and the peptide is useful for HBV-vaccine antigens.
日本臨床免疫学会の論文