嫌気性ル-メン真菌Piromyces sp.OTS3およびNeocallimastix sp.OTS4の尿素分解活性の欠如〔英文〕

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Two anaerobic rumen fungi Piromyces sp. OTS3 and Neocallimastix sp. OTS4 isolated from a goat were tested for their ability to degrade urea. The fungal cultures were not contaminated with mycoplasmas. Replacement of the trypticase and yeast extract components of Medium 10 with urea decreased the growth of Piromyces sp. OTS3 the same as the growth in Medium 10 without trypticase and yeast extract. Urea degradation was not demonstrated in the cultures of Piromyces sp. OTS3 or Neocallimastix sp. OTS4. Fungal growth and urea concentration in the medium were not correlated for Piromyces sp. OTS3 (r=0.052) or Necallimastix sp. OTS 4(r=0.324). However, fungal growth and ammonia concentration in the medium were correlated for Piromyces sp. OTS3 (r=-0.838) and Neocallimastix sp. OTS4 (r=-0.889). No ammonia was produced from urea by any washed cells of Piromyces sp. OTS3. Piromyces sp. OTS3 homo-genates did not degrade urea and did not produce ammonia from urea. These results suggest that Piromyces sp. OTS 3 and Neocallimastix sp. OTS4 have no ureolytic activity.
社団法人日本畜産学会の論文