葉緑体DNA制限酵素切断パターンのチャ及び近縁種における差異

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Five grams of mature leaves of Camellia sinensis (A 6, Kan 6, Ck 17, Cp 1, Yabukita, and Yamatomidori) and C. taliensis were homogenized in a Tris buffer in liquid nitrogen. Crude chloroplast preparations were resolved in CsC1-ethidium bromide solution and centrifuged by 45000 rpm for 36 hr. Chloroplast DNA fractions were recovered and digested by a restriction endonuclease, BamHI. After the cleaved fragments were electrophoresed on agarose gel, they were transferred to a nitrocellulose membrane and hybridized with 32P-tabacco chloroplast DNA.<BR>BamHI digestion in C. sinensis chloroplast DNA produced at least 22 fragments and the total length was appoximately 130 Kbp. C. sinensis had two unique fragments (6.2 and 3.6 Kbp) but C. taliensis did not. One of the hybrid of Assam varieties, A 6 also seemed not to have a fragment of 3.6 Kbp. These results indicate that the measurement of restriction fragment patterns of chloroplast DNAs in Camellia is useful for a genetic analysis.
日本茶業技術協会の論文