茶葉からの細胞単離とその光合成特性

概要

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Intact mesophyll cells were isolated from tea (Camellia sinensis) leaves using an enzymatic (macerozyme) technique. About 15% of the leaf cells on a chlorophyll basis from 1 or 2 grams of leaves could be isolated in 3 hours. The optimal concentration of sorbitol was 1.0 M in the case of single cells isolated from immature leaves and 0.4 M in the case of mature leaves. Cells were slightly stable when stored at 0℃. Cells isolated from immature leaves showed higher photosynthetic activity (20-30 μmoles/mg chl/hr) than that of cells isolated from mature leaves (6-10 μmoles/mg chl/hr). Photosynthetic rate of cells (immature leaves) was saturated at 5 mM of bicarbonate and the Km(CO_2) for cell photosynthesis was about 40 μM. The light intensity at maximal velocity of CO_2 fixation was about 40 klx. The optimal pH for CO_2 fixation was 7.8 and the optimal temperature was between 30 and 35℃. These results were discussed in relation to leaf photosynthesis.
日本作物学会の論文
1979-09-30