Evaluation of Rapid Antigen Detection Kits for Diagnosis of Equine Rotavirus Infection
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概要
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We evaluated antigen detection kits for human rotavirus with regard to their usefulness for diagnosing equine rotavirus infection. Limiting dilution analyses showed that of the seven kits investigated the Dipstick Eiken Rota (Dipstick) had the highest sensitivity to two serotypes of equine rotavirus. The Dipstick did not cross-react with several equine intestinal pathogens. An investigation using 249 fecal samples indicated that the sensitivity of the Dipstick was 81.9 and 47.3%, its specificity was 98.2 and 99.0%, and its concordance rate was 92.8 and 68.3%, compared with values obtained using reverse transcription polymerase chain reaction and reverse transcription loop-mediated isothermal amplification, respectively. Although a negative result does not preclude the possibility of equine rotavirus infection, the Dipstick would be useful as routine test for diagnosing equine rotavirus infection in daily clinical practice because of its ease of handling.
著者
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IMAGAWA Hiroshi
Epizootic Research Station, Equine Research Institute, Japan Racing Association
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YAMANAKA Takashi
Epizootic Research Center, Equine Research Institute, Japan Racing Association
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NIWA Hidekazu
Epizootic Research Center, Equine Research Institute, Japan Racing Association
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Tsujimura Koji
Epizootic Research Center Equine Research Institute The Japan Racing Association
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Matsumura Tomio
Epizootic Research Station Equine Research Institute The Japan Racing Association
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Matsumura Tomio
Epizootic Research Center Equine Research Institute Jra
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Imagawa Hiroshi
Epizootic Research Station Equine Research Institute Japan Racing Association
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Yamanaka Takashi
Epizootic Research Center Equine Research Institute Japan Racing Association
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KONDO Takashi
Epizootic Research Station, Equine Research Institute, Japan Racing Association
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HIGUCHI Tohru
Mitsuishi Animal Clinic Center, Hidaka Agriculture Mutual Aid Association
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Higuchi Tohru
Mitsuishi Animal Clinic Center Agriculture Mutual Aid Association Of Hidaka District
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NEMOTO Manabu
Epizootic Research Center, Equine Research Institute, Japan Racing Association
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HATA Hideaki
Mitsuishi Animal Clinic Center, Hidaka Agriculture Mutual Aid Association
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BANNAI Hiroshi
Epizootic Research Center, Equine Research Institute, Japan Racing Association
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Niwa Hidekazu
Epizootic Research Center Equine Research Institute Japan Racing Association
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Kondo Takashi
Epizootic Research Center Equine Research Institute Japan Racing Association
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Imagawa Hiroshi
Epizootic Research Center Equine Research Institute Japan Racing Association
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Bannai Hiroshi
Epizootic Research Center Eri Jra
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Nemoto Manabu
Epizootic Research Center Eri Jra
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Imagawa Hiroshi
Epizootic Research Center, Equine Research Institute, Japan Racing Association
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