Development of a Focus-Reduction Neutralizing test for Detecting Equine Herpesvirus Type-1-Neutralizing Antibodies
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概要
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Virus-neutralizing (VN) testing is essential for evaluating virus-specific immunity in equine herpesvirus type-1 (EHV-1) infection. We developed a focus-reduction neutralization test (FRNT) for EHV-1 using 96-well plates for faster large-scale testing with sufficient sensitivity. We used an overlay medium containing Avicel (FMC Biopolymer), a microcrystalline cellulose with lower viscosity than the methylcellulose. The foci were visualized by immuno-staining with anti-EHV-1 gp14 monoclonal antibody. The FRNT successfully detected seroconversion in horses experimentally infected with EHV-1 (n=3) and in those infected naturally (n=16). The FRNT for EHV-1 was high-throughput and time saving. The FRNT can be used in large-scale seroepidemiological studies of EHV-1 and in evaluating vaccine efficacy.
著者
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Tsujimura Koji
Epizootic Research Center Equine Research Institute The Japan Racing Association
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Matsumura Tomio
Epizootic Research Center Equine Research Institute Jra
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Yamanaka Takashi
Epizootic Research Center Equine Research Institute Japan Racing Association
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Kondo Takashi
Epizootic Research Center Equine Research Institute Japan Racing Association
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Bannai Hiroshi
Epizootic Research Center Eri Jra
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Nemoto Manabu
Epizootic Research Center Eri Jra
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KONDO Takashi
Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan
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