Direct Detection of Equine Herpesvirus Type 1 DNA in Nasal Swabs by Loop-Mediated Isothermal Amplification (LAMP)
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概要
- 論文の詳細を見る
We evaluated loop-mediated isothermal amplification (LAMP) as a means of detecting equine herpesvirus type 1 (EHV-1) DNA directly from nasal swabs. To increase the sensitivity, we added a step in which the samples were heat-treated to the original LAMP procedure. The detection limit of the LAMP assay with heat treatment was 10 times more sensitive than the original LAMP assay even when the DNA extraction step was omitted. In addition, the LAMP assay with heat treatment was more sensitive than the original LAMP assay and the polymerase chain reaction using clinical samples. The LAMP assay with heat treatment is easy to perform and so should be applicable to the diagnosis of EHV-1 infections in clinical laboratories.
著者
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Tsujimura Koji
Epizootic Research Center Equine Research Institute The Japan Racing Association
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Matsumura Tomio
Epizootic Research Center Equine Research Institute Jra
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Yamanaka Takashi
Epizootic Research Center Equine Research Institute Japan Racing Association
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OHTA Minoru
Racehorse Clinic, Ritto Training Center, Japan Racing Association (JRA)
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KONDO Takashi
Epizootic Research Station, Equine Research Institute, Japan Racing Association
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NEMOTO Manabu
Epizootic Research Center, Equine Research Institute, Japan Racing Association
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BANNAI Hiroshi
Epizootic Research Center, Equine Research Institute, Japan Racing Association
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Kondo Takashi
Epizootic Research Center Equine Research Institute Japan Racing Association
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Bannai Hiroshi
Epizootic Research Center Eri Jra
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Nemoto Manabu
Epizootic Research Center Eri Jra
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Ohta Minoru
Racehorse Clinic Ritto Training Center Japan Racing Association (jra)
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TSUJIMURA Koji
Epizootic Research Center, Equine Research Institute, Japan Racing Association
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