Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Assay for H7N7 Equine Influenza Virus
スポンサーリンク
概要
- 論文の詳細を見る
We have developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting H7N7 equine influenza virus (EIV). The detection limit of the RT-LAMP assay was a virus dilution of 10-4, which was 10 times more sensitive than that of Espline Influenza A-N and the same as that of reverse transcription polymerase chain reaction. The RT-LAMP assay specifically amplified H7N7 EIV strains but did not amplify several pathogens related to equine respiratory disease including H3N8 EIV strains. Because it provides ease of manipulation, the RT-LAMP assay is suitable for large-scale surveillance for H7N7 EIV. In addition, the combination of the H3N8 RT-LAMP assay, which was developed previously, with the H7N7 RT-LAMP assay should be useful to discriminate between H3N8 and H7N7 EIVs in clinical laboratories.
著者
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Tsujimura Koji
Epizootic Research Center Equine Research Institute The Japan Racing Association
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Matsumura Tomio
Epizootic Research Center Equine Research Institute Jra
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Yamanaka Takashi
Epizootic Research Center Equine Research Institute Japan Racing Association
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Kondo Takashi
Epizootic Research Center Equine Research Institute Japan Racing Association
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Bannai Hiroshi
Epizootic Research Center Eri Jra
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Nemoto Manabu
Epizootic Research Center Eri Jra
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NEMOTO Manabu
Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan
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YAMANAKA Takashi
Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan
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TSUJIMURA Koji
Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan
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BANNAI Hiroshi
Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan
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MATSUMURA Tomio
Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan
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KONDO Takashi
Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan
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