ウシ子宮内膜上皮細胞の分離とその無血清培養
スポンサーリンク
概要
- 論文の詳細を見る
The objective of this study was to establish a serum-free culture system for bovine endometrial epithelial cells, and to identify the function of these cells. Epithelial cells were separated from uterine endometrium at Days 5-10 of the estrous cycle by 0.76% EDTA-PBS and collagenase treatment. After histological observation, the epithelium was completely separated from the basement membrane by EDTA treatment. The separated epithelial cells were then dispersed by collagenase and cultured in Dulbeccos Modified Eagles Medium/Hams F-12 1:1 supplemented with insulin, transferrin, sodium-selenite, hydrocortisone, retinol, ascorbic acid and antibiotics on collagen-coated culture dishes. The cells attached to the substrate and reached confluency after 5-7 days of culture. An immunocytochemical study revealed that the cultured epithelial cells were positive to cytokeratin, but not vimentin. The production of prostaglandin (PG) F2 α was significantly higher (p<0.001) in the presence of 100 nM oxytocin (OT; 2.81 fold greater than the concentration in the control) than in the absence of OT. These results indicate that it is possible to culture bovine endometrial epithelial cells in the serum-free culture system, and that cultured cells have some typical characteristics of endometrial epithelial cells.
- 日本繁殖生物学会の論文
著者
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TAKAHASHI Masashi
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The U
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高橋 ひとみ
Department of Animal Reproduction, National Institute of Animal Industry
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OKANO Akira
Department of Animal Reproduction, National Institute of Animal Industry
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伊賀 皓輔
Department of Animal Reproduction, National Institute of Animal Industry
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佐藤 太郎
Livestock Research Center, Niigata Prefectural Agricultural Research Institute
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高橋 ひとみ
農林水産省畜産試験場繁殖部
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Takahashi Masashi
Department Of Chemistry Faculty Of Science Toho University
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Sato Taro
Livestock Research Center Niigata Agricultural Research Institute
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Takahashi Hitomi
Department Of Mechatronics And Precision Engineering
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IGA Kosuke
Department of Animal Reproduction, National Institute of Animal Industry
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Takahashi Masashi
Department Of Animal And Grassland Research National Agricultural Center For Kyushu Okinawa Region
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