Estradio1-17β and Progesterone Synthesis in the Trophoblastic Vesicles Formed <I>in Vitro</I> by the Conceptuses Obtained from Day-14 Pregnant Pigs
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概要
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The elongated conceptuses were collected from pregnant sows on Day 14 by flushing uteri with PBS. Conceptuses cultured <I>in vitro</I> in M-199 at 37C, under an atmosphere of 5% CO<SUB>2</SUB> in air, formed trophoblastic vesicles by the following day. The trophoblastic vesicles were cultured continuously in vitro and subjected to successive subculture, where the free-floating trophoblastic cells again formed small trophoblastic vesicles. The trophoblastic vesicles of all stages of culture showed similar patterns of the protein synthesis. The elongated conceptus and trophoblastic vesicle synthesized α-fetoprotein and retinol-binding protein. While culturing trophoblastic vesicles <I>in vitro</I>, fairly large amounts of estradiol-17β (E<SUB>2</SUB>) and progesterone were synthesized. Possibilities that E<SUB>2</SUB> acts as a signal for the recoginition of pregnancy were discussed.
- 日本繁殖生物学会の論文
著者
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OKANO Akira
Department of Animal Reproduction, National Institute of Animal Industry
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Takahashi Masashi
Department Of Animal And Grassland Research National Agricultural Center For Kyushu Okinawa Region
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