Tumor Necrosis Factor-.ALPHA. Induces Apoptosis in Cultured Porcine Luteal Cells
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概要
- 論文の詳細を見る
Some studies in mammalian species recently demonstrated that tumor necrosis factor (TNF)-αplays an important role for corpus luteum (CL) function by way of apoptosis during the estrous cycle. The objectives of this study were to clarify the induction of apoptosis in cultured porcine luteal cells by TNF-α treatment. Luteal cells prepared from porcine ovaries collected from crossbred mature gilts on Days 10-14 of the estrous cycle were isolated and examined as follows: 1) Flow cytometric analysis was carried out to determine apoptosis in cultured luteal cells. 2) Single-cell gel electrophoresis (comet assay) was performed to investigate apoptotic DNA fragmentation in luteal cells. The results of the flow cytometric analysis and comet assay demonstrated coincidentally that TNF-α induces DNA fragmentation in luteal cells causing apoptosis. These results revealed that TNF-α is an inducing factor of apoptosis in luteal cells.
- 日本繁殖生物学会の論文
著者
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OKANO Akira
Department of Animal Reproduction, National Institute of Animal Industry
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Takahashi Hitomi
Department Of Mechatronics And Precision Engineering
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Takahashi Masashi
Department Of Animal And Grassland Research National Agricultural Center For Kyushu Okinawa Region
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KISHI Hisashi
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Sciences
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TAKAHASHI Hitomi
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
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TAKAHASHI Hitomi
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Sciences
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OKANO Akira
Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science
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