Inhibitory effects of catechin derivatives on mammalian DNA polymerase and topoisomerase activities and mouse one-cell zygote development(BIOCHEMICAL ENGINEERING)
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概要
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In this study, the inhibitory activities against DNA polyinerases (pols) and DNA topoisomerases (topos) by eight major green tea catechin derivatives (flavan-3-ols) were investigated. Some catechins inhibited mammalian pols (α and β) and human topos (I and II), with (-)-epigallocatechin gallate (EGCg) the strongest inhibitor of both enzyme types, showing IC_<50> values of 3.8-21.5 and 2.0-20.0 μM, respectively. EGCg did not affect the activities of plant (cauliflower) pol α or prokaryotic pols and showed no effect on the activities of other DNA metabolic enzymes tested. Next, a method was established for assay of mouse one-cell zygote development inhibition, the catechin derivatives screened for bioactivity, and the inhibition was assessed and their effects ranked as: EGCg > GCg > Cg ≫ others. In the mouse one-cell zygote assay, EGCg at 50 μM increased abnormal cells and 75 μM of EGCg-induced apoptosis. The observed ranking of catechin derivative inhibition effects against mouse one-cell zygote development in vivo was similar to their ranking by topo inhibition in vitro rather than by pol inhibition; therefore, topo inhibition might have been effecting zygote development inhibition. These results suggested that catechin derivatives indeed reached the nuclear DNA where topo inhibition can occur, thus causing the observed cellular effects. From these findings, this zygote development inhibition assay will be useful as an anti-pregnant agent screening.
- 2013-03-00
著者
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KURIYAMA Isoko
Laboratory of Food & Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University
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YOSHIDA Hiromi
Laboratory of Food & Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University
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MIZUSHINA Yoshiyuki
Laboratory of Food & Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University
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Yoshida Naoko
Department Of Biomedical Sciences College Of Life Sciences Ritsumeikan University
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YOSHIDA Hiromi
Laboratory of Food & Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University
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Mizushina Yoshiyuki
Laboratory of Food & Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University:Cooperative Research Center of Life Sciences, Kobe-Gakuin University
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Kuriyama Isoko
Laboratory of Food & Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University
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