MNM-P9-1 接触法によるチャネル膜たんぱく質のマルチアレイ化(P9 マイクロ・ナノスケールバイオ計測と医用応用)

概要

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This paper describes simultaneous ion-channel recordings through membrane proteins reconstituted in bilayer lipid membranes (BLMs) array. The BLMs array can be prepared by a contact method of lipid monolayers. We found that the BLMs were formed readily by a "Droplets-Contacting-Method". This method is one of the easiest ways for preparing BMLs because it is reproducible, easy to apply to automated system, and useless skilled techniques. We used a double-well chamber for this method and prepared BLMs with 2 steps. Step 1) The entire chamber was filled with 15 μL of n-decane. Step 2) Two droplets of buffer solution (15 μL each) were spotted into each well. Then the BLMs were formed at the boundary after a few minutes. We can record the protein channel signal by introducing Ag/AgCl electrodes into the buffer. This method can be applied to fully automated BLMs array formation for high-throughput screening system using a spotting robot.
一般社団法人日本機械学会の論文
2010-10-12