T1501-2-6 肝細胞スフェロイド3次元形成のためのマイクロ旋回流生成デバイスの開発(マイクロ・ナノ技術のロボティクス応用(2))

概要

論文の詳細を見る
Hepatocytes are responsible for metabolic and detoxification processes in the liver. During the tissue formation from isolated cells, they increase the cell density and acquire their liver-specific functions, new protein expression and cell signaling. Hence, tissue-based in vitro studies are strongly demanded. Spheroids are promising research target for this purpose that are aggregates of cells and retain three dimensional structures and tissue-specific functions. Recently several methods of forming spheroids were reported. However, they are not capable of controlling the geometry of spheroids. We propose a three dimensional spheroids of hepatocytes formation platform using micro-swirling flow. The flow can control the spheroid formation speed and the three dimensional geometry. Such three dimensional spheroids formation platform will not offer only new experimental systems that imitate tissues for biological research but also efficient drug screening systems and hybrid artificial organs with high cell density and hepatic functions.
2009-09-12