Random Amplified Polymorphic DNA and Restriction Fragment Length Polymorphism analyses of Cannabis sativa
スポンサーリンク
概要
- 論文の詳細を見る
Random Amplified Polymorphic DNA (RAPD) and Restriction Fragment Length Polymorphism (RFLP) methods were used for the analysis of three different strains of Cannabis sativa that included the Mexican (drug type), Tochigishiro (fiber type) and Nara cultivated strains. Cannabis sativa samples were grouped into three chemotypes, drug type, fiber type and intermediate drug type by GC analysis. The RAPD analysis was able to distinguish the three chemotypes clearly, however, in each type, some genetic diversities was shown. The RFLP analysis showed that the genetic distance between the Tochigishiro and Nara cultivated strains was small, whereas that between the Mexican strain and the other two strains was large, and that there was no genetic diversity within each strain. These results indicated that the RAPD and RFLP analyses, using DNA as a marker, applied in combination might be useful for distinguishing between the strains having chemotypes.
- 日本生薬学会の論文
- 1998-04-20
著者
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Yamazaki Mami
Laboratory Of Cell Biology Department Of Regulation Biology Faculty Of Science Saitama University
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Shirota Osamu
Division Of Pharmacognosy Phytochemistry And Narcotics National Institute Of Health Sciences
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Shirota Osamu
Shiseido Research Center
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Sekita Setsuko
大阪府立公衆衛生研究所
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Sekita Setsuko
Division Of Pharmacognosy And Phytochemistry National Institute Of Health Sciences
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Sekita Setsuko
湧永製薬ヘルスケア研究所
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Ohta Sanae
Laboratory Of Pharmacognosy And Natural Product Chemistry Faculty Of Pharmaceutical Sciences At Kaga
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Watanabe Akiko
Laboratory Of Molecular Biology And Biotechnology Research Center Of Medicinal Resources Faculty Of
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Watanabe Akiko
Laboratory Of Entomology Faculty Of Agriculture Hirosaki University
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Saito Kazuki
Laboratory Of Molecular Biology And Biotechnology Research Center Of Medicinal Resources Faculty Of
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Shibano Katsunari
Kinki Regional Narcotics Control Office
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Satake Notoyoshi
Division of Pharmacognosy and Phyochemistry, National Institute of Health Sciences
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Satake Notoyoshi
Division Of Pharmacognosy And Phyochemistry National Institute Of Health Sciences
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SHIROTA Osamu
Division of Pharmacognosy and Phytochemistry, National Institute of Health Sciences
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