Studies on "Signal" Constituents for Evaluation of Animal Crude Drugs. IV : Application of DNA analytical technique to quality evaluation of medicines containing animal crude drugs

概要

論文の詳細を見る
For the quality evaluation of medicines containing animal crude drugs, Cervi Parvum Cornu, Agkistrodon Japonicae and Hippocampus, histological examination and PCRs using four sets of primers, amplifying the DNA fragments of 12S rRNA gene region and/or of cytochrome b gene region, were performed. The presence of DNA in the tissues, and the reproducibility of PCR products were confirmed in all the animal crude drug - containing medicinal preparations. The PCR products of Cervi Parvum Cornu, Agkistrodon Japonicae, and Hippocampus, amplified by using the same primer set, were clearly separated by electrophoresis. This suggested the possibility that each animal crude drug in the medicines can be identified simultaneously by the comparison of the PCR products with each reference standard sample.
日本生薬学会の論文
1998-02-20