J0201-2-5 前臨床試験を目指した肝転移モデルの非侵襲的観察(ドラッグデリバリーシステムとその周辺技術の基礎と展開(2))

概要

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Preclinical mouse models are essential to the study of liver metastasis, yet their utility has been limited by difficulty in tracking the progression of metastases through time. In this study, we attempted to applicate some non-invasive methods to study the murine liver metastasis. Livers of mice were observed by camera system termed photo dynamic eye (PDE) with the indocianine green (ICG) injected into the tail vein. Mice received and intrasplenic injection of luciferase-expressing tumor cells. Luciferase activity was measured by in vivo bioluminescence system (IVIS). Ultrasound imaging was performed by a high-frequency ultrasound system (VEVO), after nanobubbles were administered from the tail vein. As the result, murine liver metastases were identified using PDE and IVIS. Two-dimensional and three dimensional images by ultrasound and nanobubbles showed vessels in the liver. These results could be benefit for early diagnosis of liver metastases with a minimum detection size in preclinical model.
社団法人日本機械学会の論文
2009-09-12