高感度インスリン測定法の開発と尿中及び乾燥ろ紙血中インスリンによる糖尿病リスク指標の構築

概要

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We developed highly sensitive enzyme immunoassay for insulin that allowed the measurementof insulin in urine and dried blood on filter paper. As a result of having comparedurinary insulin with blood insulin after OGTT, serum insulin levels by dried blood on filterpaper at 60 minutes were significantly correlated with total urinary insulin value during120 minutes (p<0.01).Subsequently we compared an insulin level in the morning urine from three groups ofnon-diabetic non-obese subjects, non-diabetic obese subjects and the diabetic patients,respectively. The total insulin value in urine of the non-diabetic obese subjects was significantlyhigher than both of non-diabetic non-obese subjects and the diabetic patients(p<0.05). On the other hand, by the correction with the urinary creatinine, the increasethat was more significant than non-diabetic non-obese subjects was found in both of nondiabeticobese subjects and the diabetic patients (p<0.01).From these results, urinary insulin in the morning is a good marker for the risk of diabetesmellitus and may be effective in determining which patients need exercise guidanceand nutritional education.
徳島文理大学の論文