Quantitative Analysis of Cytokine mRNA Expression and Protozoan DNA Load in Theileria parva-Infected Cattle(Immunology)
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概要
- 論文の詳細を見る
Theileria parva (T. parva) causes a highly serious bovine disease called East Coast fever (ECF), which is characterlzed by pyrexia, dyspnea and cachexia and is of great economic importance in African countries. We hypothesize that the clinical symptoms of ECF could be explained by a cytokine dysregulation. In this study, we investigated the relationship between T. parva DNA load and expression levels of cytokine mRNAs in leukocytes from experimentally infected calves by quantitative PCR. The p104 gene, which encodes the T. parva 104kDa microneme-rhoptry protein, was detected in cattle blood from day 10 after T. parva-infected tick infestation, and the protozoan DNA load was increased together with severity of disease. The mRNA expressions of pro-inflammatory cytokines, such as interleukin (IL)-1β and IL-6, were up-regulated with protozoan DNA load increasing. In addition, the level of a type-2 cytokine (IL-10) transcript was also increased during the acute phase. In contrast, the down-regulation or no detectable levels of the expression of type-1 cytokines, such as IL-2 and interferon (IFN)-γ were observed in T. parva-infected animals. Thus, our observations indicated that high protozoan load and resulting intense inflammatory responses might be involved ill the severity of clinical signs observed in T. parva-infection.
- 社団法人日本獣医学会の論文
- 2009-01-25
著者
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ONUMA Misao
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University
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OHASHI Kazuhiko
Department of Gastroenterology, Aichi Cancer Center Hospital
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Okuma Katashi
Department Of Clinical Laboratory Aichi Cancer Center Hospital
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Ohashi Kazuhiko
Hokkaido Univ. Sapporo Jpn
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Ohashi Kazuhiko
Laboratory Of Infectious Diseases Department Of Disease Control Graduate School Of Veterinary Medici
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Ohashi K
Laboratory Of Infectious Diseases Graduate School Of Veterinary Medicine Hokkaido University
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Simuunza Martin
Epidemiology Section Disease Control Department School Of Veterinary Medicine University Of Zambia
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Chembensofu Mwelwa
Epidemiology Section Disease Control Department School Of Veterinary Medicine University Of Zambia
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Konnai Satoru
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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YAMADA Shinji
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
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IMAMURA Saiki
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
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CHOTA Amos
Epidemiology Section, Disease Control Department, School of Veterinary Medicine, University of Zambi
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NAMBOTA Andrew
Epidemiology Section, Disease Control Department, School of Veterinary Medicine, University of Zambi
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Chota Amos
Epidemiology Section Disease Control Department School Of Veterinary Medicine University Of Zambia
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Onuma Misao
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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Onuma M
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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Imamura Saiki
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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Nambota Andrew
Epidemiology Section Disease Control Department School Of Veterinary Medicine University Of Zambia
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Yamada Shinji
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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Ohashi Kazuhiko
Laboratory Of Infectious Disease Graduate School Of Veterinary Medicine Hokkaido University
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Ohashi Kazuhiko
Department Of Diagnostic Radiology Aichi Cancer Center Hospital
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Yamada Shinji
Department Of Chemistry Faculty Of Science Ochanomizu University
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Ohashi Kazuhiko
Department Of Chemistry Faculty Of Science Kyushu University
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