Virological and Serological Studies of Porcine Respiratory Coronavirus Infection on a Japanese Farm
スポンサーリンク
概要
- 論文の詳細を見る
The prevalence of trypanosome infections in tsetse flies, Glossina pallidipes, collected from Chiawa and Chakwenga in Zambia with endemic trypanosomosis was assessed by polymerase chain reaction (PCR). Out of the 550 G. pallidipes, 58 (10.5%) flies were found to harbor trypanosome DNA. Infection rates of tsetse with Trypanosoma vivax universal, Trypanosoma congolense savannah, T. congolense forest and T. congolense kilifi were 4.2% (23/550), 4.7% (26/550), 1.1% (6/550) and 1.6% (9/550), respectively. To determine the mammalian hosts of T. congolense and T. vivax infections from the tsetse flies, mammalian mitochondrion DNA of blood meal in these flies were analyzed by PCR and subsequent gene sequence analysis of the amplicons. Sequence analysis showed the presence of cytochrome b gene (cyt b) of 7 different mammalian species such as human, elephant, buffalo, goat, warthog, greater kudu and cattle. Goats which were main livestock in these areas were further examined to know the extent of its contribution in spreading the infection. We examined the prevalence of trypanosome infections in the domestic goat population in 6 settlements in Chiawa alone. Of the 86 goats sampled, 4 (4.6%), 5 (5.8%), 4 (4.6%) and 4 (4.6%) were positive for T. vivax universal, T. congolense savannah, forest and kilifi, respectively. These findings showed that the host-source of trypanosome infections in vector fly give a vital information about spread of infection. The result of this study will certainly contribute in elucidating more the epidemiology of trypanosomosis.
著者
-
Simuunza Martin
Epidemiology Section Disease Control Department School Of Veterinary Medicine University Of Zambia
-
Chembensofu Mwelwa
Epidemiology Section Disease Control Department School Of Veterinary Medicine University Of Zambia
-
Konnai Satoru
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
-
Onuma Misao
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
-
Inoue Noboru
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
-
Ohashi Kazuhiko
Department Of Chemistry Faculty Of Science Kyushu University
-
Kano Rika
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
-
MEKATA Hirohisa
Department of Disease Control, School of Veterinary Medicine, Hokkaido University
-
WITOLA William
Department of Disease Control, School of Veterinary Medicine, Hokkaido University
-
TEMBO Mwase
Epidemiology Section, Disease Control Department, School of Veterinary Medicine, University of Zambia
-
CHITAMBO Harrison
Epidemiology Section, Disease Control Department, School of Veterinary Medicine, University of Zambia
-
ONUMA Misao
Department of Disease Control, School of Veterinary Medicine, Hokkaido University
-
OHASHI Kazuhiko
Department of Disease Control, School of Veterinary Medicine, Hokkaido University
-
KANO Rika
Department of Basic Veterinary Science, School of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine
-
KANO Rika
Department of Disease Control, School of Veterinary Medicine, Hokkaido University
関連論文
- ザンビア共和国におけるトリパノソーマ病の疫学調査と伝播経路の探索(寄生虫病学)
- 吸血中のフタトゲチマダニ(Haemaphysalis longicornis)唾液腺由来cDNAライブラリーによる発現遺伝子の網羅的解析(免疫学)
- 特集 増加傾向にある牛白血病の現状と対策(2)診療・生産現場からの声に対して
- Brucella abortus S19ワクチン接種ラクダにおけるサイトカインプロファイル解析(免疫学)
- フタコブラクダ(Camelus bactrianus) Th1およびTh2型サイトカイン遺伝子の同定(免疫学)
- アポトーシスを誘導した鶏腫瘍細胞株における欠損型p53の発現(家禽疾病学)
- フタトゲチマダニ(Haemaphysalis longicornis)の唾液腺由来cDNAライブラリーより得られた新規のトロンビン阻害蛋白質をコードする遺伝子(免疫学)
- ファージディスプレイ法によるニューカッスル病ウイルス結合性ペプチドの同定およびその性状解析(ウイルス学)
- BLV tax遺伝子分子クローン接種ヒツジにおけるリンパ腫の発症(病理学)
- ラクダ科動物(ラマ, ラクダ)炎症性サイトカイン遺伝子の同定および系統樹解析(免疫学)