Effects of anti-tick cocktail vaccine against Rhipicephalus appendiculatus
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概要
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Rhipicephalus appendiculatus serpin-3 (RAS-3), R. appendiculatus serpin-4 (RAS-4) and a 36-kDa immuno-dominant protein of R. appendiculatus (RIM36) were reported as candidate antigens for the anti-tick vaccine to control ixodid ticks. In the present study, we generated recombinant proteins of RAS-3 (rRAS-3), RAS-4 (rRAS-4) and RIM36 (rRIM36), and assessed their potency as an anti-tick cocktail vaccine in cattle model. RT-PCR analysis showed that RAS-3, RAS-4 and RIM36 transcripts were detected in both adult male and female ticks during feeding. Immunization of cattle with the combination of rRAS-3, rRAS-4 and rRIM36 had raised antibodies against all recombinants and anti-sera had reacted with the molecules from the tick salivary gland extract. Tick infestation challenge demonstrated protective immunity against female ticks, resulting in mortality rates of 39.5 and 12.8 % for the vaccinated and control groups, respectively. Moreover, the mortality rate of Theileria parva-infected female ticks was 48.5 and 10.8 % in the vaccinated and control group, respectively. In order to evaluate the levels of pathogen transmission capacity by T. parva-infected ticks fed on immunized cattle, the occurrence of T. parva in the bovine parotid lymph node and peripheral blood was also determined and quantified by real-time PCR. Although the infection with T. parva could not be protected by the vaccine, the occurrence of pathogen in peripheral blood was delayed 1 to 2 days after the infestation challenge in vaccinated group. These results suggest that this cocktail vaccine plays a role in the prevention of tick infestation.
- The Graduate School of Veterinary Medicine, Hokkaido Universityの論文
著者
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Yasuda J
北海道大学 獣医学研究科感染症学教室
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Okuma Katashi
Department Of Clinical Laboratory Aichi Cancer Center Hospital
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Ohashi Kazuhiko
Hokkaido Univ. Sapporo Jpn
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Ohashi Kazuhiko
Laboratory Of Infectious Diseases Department Of Disease Control Graduate School Of Veterinary Medici
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Ohashi K
Laboratory Of Infectious Diseases Graduate School Of Veterinary Medicine Hokkaido University
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Simuunza Martin
Epidemiology Section Disease Control Department School Of Veterinary Medicine University Of Zambia
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Konnai Satoru
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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YAMADA Shinji
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
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Onuma M
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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Imamura Saiki
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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Yamada Shinji
Department Of Disease Control Graduate School Of Veterinary Medicine Hokkaido University
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Ohashi Kazuhiko
Laboratory Of Infectious Disease Graduate School Of Veterinary Medicine Hokkaido University
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