A Field study to Estimate the Prevalence of Bovine African Trypanosomosis in Butaleja District, Uganda
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概要
- 論文の詳細を見る
Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results suggests that the most prevalent trypanosome was T. b. brucei (31/203), followed by T. congolense (6/203). In addition, only a few (3/203) mixed infections of T. b. brucei and T. congolense was detected by the PCR. Results obtained from this study indicates that bovine trypanosomosis is endemic in Butaleja district, Uganda.
- 社団法人 日本獣医学会の論文
著者
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Sakurai Tatsuya
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
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Inoue Noboru
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
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THEKISOE Oriel
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Me
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SUGIMOTO Chihiro
Research Center for Zoonosis Control, Hokkaido University
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JING Zhang
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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MAGONA Joseph
Research Center for Zoonosis Control, Hokkaido University
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OTIM Charles
National Livestock Resources Research Institute (NaLIRRI)
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