Overproduction of the Pro-Apoptotic Molecule, Programmed Cell Death 5, in Toxoplasma gondii Leads to Increased Apoptosis of Host Macrophages
スポンサーリンク
概要
- 論文の詳細を見る
We established a recombinant strain of iToxoplasma gondii/i that overexpressed programmed cell death 5 (TgPDCD5), in order to evaluate the role of endogenous TgPDCD5 in macrophage apoptosis during iT. gondii/i infection. Immunofluorescence microscopy revealed that overproduced TgPDCD5 with a hemagglutinin tag was localized in the cytosol, which was consistent with the localization of endogenous TgPDCD5. The induced TgPDCD5-HA was recognized as an additional band by Western blot analysis, indicating successful overexpression of TgPDCD5. Secretion and release of TgPDCD5 by the parasite was also up-regulated in a time-dependent manner, which reflected its overproduction. Apoptosis due to parasite infection and interferon-gamma treatment was significantly up-regulated by the overexpression of TgPDCD5. These results suggest that endogenous TgPDCD5 plays a role in macrophage apoptosis during iT. gondii/i infection.br
著者
-
IBRAHIM Hany
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Me
-
YAMADA Kyoko
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Me
-
Xuan Xuenan
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
-
SUGIMOTO Chihiro
Research Center for Zoonosis Control, Hokkaido University
-
Kawase Osamu
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
-
Bannai Hiroshi
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
-
NISHIKAWA Yoshifumi
National Research Center for Protozoan Deseases, Obihiro University of Agriculture and Veterinary Medicine
-
WATANABE Jun-ichi
The Institute of Medical Science, The University of Tokyo
-
IBRAHIM Hany
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
-
XUAN Xuenan
National Research Center for Protozoan Deseases, Obihiro University of Agriculture and Veterinary Medicine
関連論文
- Roles of CD122+ Cells in Resistance against Neospora caninum Infection in a Murine Model
- Characterization of the Babesia gibsoni 12-kDa protein as a potential antigen for the serodiagnosis
- Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates
- Genotypic Diversity of Theileria orientalis Detected from Cattle Grazing in Kumamoto and Okinawa Prefectures of Japan
- Seroprevalence of Bartonella henselae, Toxoplasma gondii, FIV and FeLV Infections in Domestic Cats in Japan
- Host Cholesterol Synthesis Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages
- Dissemination of extracellular and intracellular Toxoplasma gondii tachyzoites in the blood flow
- Molecular Epidemiological Survey of Theileria orientalis in Thua Thien Hue Province, Vietnam
- Starfish, Asterias amurensis and Asterina pectinifera, as Potential Sources of Th1 Immunity-Stimulating Adjuvants
- Expression Patterns of the Implantation-associated Genes in the Uterus during the Estrous Cycle in Mice
- Detection of Babesia gibsoni by Reaction of Phage Display Single Chain Antibodies with P50 Proteins
- マウスの発情周期における着床関連遺伝子の発現について
- Artesunate, a potential drug for treatment of Babesia infection
- Characterization of a novel thrombospondin-related protein in Toxoplasma gondii
- A Field study to Estimate the Prevalence of Bovine African Trypanosomosis in Butaleja District, Uganda
- Overproduction of the Pro-Apoptotic Molecule, Programmed Cell Death 5, in Toxoplasma gondii Leads to Increased Apoptosis of Host Macrophages
- Epidemiological Survey of Babesia bovis and Babesia bigemina Infections of Cattle in Philippines
- Identification, Cloning and Characterization of BmP41, a Common Antigenic Protein of Babesia microti
- Prevalence and Dynamics of Antibodies against NcSAG1 and NcGRA7 Antigens of Neospora caninum in Cattle during the Gestation Period
- Molecular Detection of Theileria Species in Sheep from Northern China
- PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam
- Molecular Cloning of Two Caspase-Like Genes from the Hard Tick Haemaphysalis longicornis
- Babesia bovis Dihydroorotate Dehydrogenase (BboDHODH) is a Novel Molecular Target of Drug for Bovine Babesiosis
- Comparison of the Growth Inhibitory Effects of Canine IFN-.ALPHA., -.BETA. AND -.GAMMA. on Canine Cells Infected with Neospora caninum Tachyzoites.
- Epidemiological Survey of Theileria Parasite Infection of Cattle in Northeast China by Allele-Specific PCR
- Epidemiological Investigation of Equine Piroplasmosis in China by Enzyme-Linked Immunosorbent Assays
- Vaccine Development against Neospora caninum Infection.