Identification, Cloning and Characterization of BmP41, a Common Antigenic Protein of Babesia microti
スポンサーリンク
概要
- 論文の詳細を見る
Babesia microti is a rodent tick-borne blood parasite and the major causative agent of emerging human babesiosis. Here, we identified a candidate of common antigenic protein BmP41 of B. microti by serological screening of cDNA library of human-pathogenic Gray strain with antisera against rodent Munich strain. Immunofluorescent antibody test using mouse anti-recombinant BmP41 (rBmP41) serum revealed that native BmP41 was expressed in each of the developmental stages of B. microti merozoites. An enzyme-linked immunosorbent assay (ELISA) using rBmP41 detected specific antibodies in sera from hamsters infected with B. microti Gray strain and mice infected with B. microti Munich strain. Taken together, BmP41 could be a promising universal serological marker for diagnosis of human babesiosis.
著者
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Xuan Xuenan
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
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Nishikawa Yoshifumi
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
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TERKAWI Mohamad
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Me
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Ooka Hideo
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
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ASADA Masahito
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
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MASATANI Tatsunori
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
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CAO Shinuo
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
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LUO Yuzi
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
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HAYASHI Kei
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
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LUO Yuzi
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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CAO Shinuo
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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NISHIKAWA Yoshifumi
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
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NISHIKAWA Yoshifumi
National Research Center for Protozoan Deseases, Obihiro University of Agriculture and Veterinary Medicine
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TERKAWI Mohamad
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
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MASATANI Tatsunori
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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XUAN Xuenan
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080–8555, Japan
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