Molecular Cloning of Two Caspase-Like Genes from the Hard Tick Haemaphysalis longicornis

スポンサーリンク

概要

• 論文の詳細を見る

• We identified two caspase-like genes from the midgut cDNA library of the hard tick, Haemaphysalis longicornis. Sequence analysis showed that these cDNAs encoded homologues of caspase-2 and caspase-8 that were categorized as apoptosis initiators. The H. longicornis caspase-2 (Hlcaspase-2) cDNA encodes 340 amino acid residues with a predicted molecular weight (Mw) of 38.5 kDa. Another cDNA identified as the H. longicornis caspase-8 (Hlcaspase-8) encodes 306 amino acid residues with an estimated Mw of 35.3 kDa. A catalytic active site was highly conserved in Hlcaspase-8 but not in Hlcaspase-2. RT-PCR analysis showed that both Hlcaspase-2 and Hlcaspase-8 were expressed in tick midgut and salivary glands. This is the first report of the molecular cloning of apoptosis-related genes in the tick.

• 公益社団法人 日本獣医学会の論文

著者

• Xuan Xuenan

  National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med

• Fujisaki Kozo

  National Institute Of Animal Health

• Tanaka Miho

  National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med

• NISHIKAWA Yoshifumi

  National Research Center for Protozoan Deseases, Obihiro University of Agriculture and Veterinary Medicine

• LIAO Min

  National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

• ZHOU Jinlin

  National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

• XUAN Xuenan

  National Research Center for Protozoan Deseases, Obihiro University of Agriculture and Veterinary Medicine

関連論文

• Roles of CD122+ Cells in Resistance against Neospora caninum Infection in a Murine Model
• Characterization of the Babesia gibsoni 12-kDa protein as a potential antigen for the serodiagnosis
• Theileria sergenti感染牛における,末梢血単球の比率および実数の変化(短報)〔英文〕
• Inhibition effect of tick defensin (longicin) on dog B. gibsoni infection(Proceedings of 14th Annual Meeting of the Acarological Society of Japan)
• Developmental Stage- and Organ-Specific Expression Profiles of Asparaginyl Endopeptidases/Legumains in the Ixodid Tick Haemaphysalis longicornis(Parasitology)
• A set of serine proteinase paralogs are required for blood-digestion in the ixodid tick Haemaphysalis longicornis
• Establishment of an in vitro transgene expression system in epimastigotes of Trypanosoma congolense
• Seroprevalence of Bartonella henselae, Toxoplasma gondii, FIV and FeLV Infections in Domestic Cats in Japan
• Host Cholesterol Synthesis Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages
• Dissemination of extracellular and intracellular Toxoplasma gondii tachyzoites in the blood flow
• Molecular Epidemiological Survey of Theileria orientalis in Thua Thien Hue Province, Vietnam
• Starfish, Asterias amurensis and Asterina pectinifera, as Potential Sources of Th1 Immunity-Stimulating Adjuvants
• Hemoglobinase activity of a cysteine protease from the ixodid tick Haemaphysalis longicornis
• Expression Patterns of the Implantation-associated Genes in the Uterus during the Estrous Cycle in Mice
• Detection of Babesia gibsoni by Reaction of Phage Display Single Chain Antibodies with P50 Proteins
• Leucine Aminopeptidase in the Ixodid Tick Haemaphysalis longicornis: Endogenous Expression Profiles in Midgut
• マウスの発情周期における着床関連遺伝子の発現について
• Artesunate, a potential drug for treatment of Babesia infection
• Characterization of a novel thrombospondin-related protein in Toxoplasma gondii
• Leucine aminopeptidase, HlLAP, from the ixodid tick Haemaphysalis longicornis, plays vital roles in the development of oocytes
• Comparison of Macrophage Scavenger Receptor-A Knockout Mice with Wild Type Ones in the Immune Response against Repeated Infestation with Haemaphysalis longicornis
• Overproduction of the Pro-Apoptotic Molecule, Programmed Cell Death 5, in Toxoplasma gondii Leads to Increased Apoptosis of Host Macrophages
• Developmental Stage- and Organ-Specific Expression Profiles of Asparaginyl Endopeptidases/Legumains in the Ixodid Tick Haemaphysalis longicornis
• Epidemiological Survey of Babesia bovis and Babesia bigemina Infections of Cattle in Philippines
• Identification, Cloning and Characterization of BmP41, a Common Antigenic Protein of Babesia microti
• Prevalence and Dynamics of Antibodies against NcSAG1 and NcGRA7 Antigens of Neospora caninum in Cattle during the Gestation Period
• Molecular Detection of Theileria Species in Sheep from Northern China
• PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam
• Genetic Diversity of Benign Theileria Parasites of Cattle in the Okinawa Prefecture
• Molecular Epidemiological Survey of Benign Theileria Parasites of Cattle in Japan: Detection of a New Type of Major Piroplasm Surface Protein Gene
• Molecular Cloning of Two Caspase-Like Genes from the Hard Tick Haemaphysalis longicornis
• Babesia bovis Dihydroorotate Dehydrogenase (BboDHODH) is a Novel Molecular Target of Drug for Bovine Babesiosis
• Comparison of the Growth Inhibitory Effects of Canine IFN-.ALPHA., -.BETA. AND -.GAMMA. on Canine Cells Infected with Neospora caninum Tachyzoites.
• Epidemiological Survey of Theileria Parasite Infection of Cattle in Northeast China by Allele-Specific PCR
• Epidemiological Investigation of Equine Piroplasmosis in China by Enzyme-Linked Immunosorbent Assays
• Vaccine Development against Neospora caninum Infection.

もっと見る 閉じる

スポンサーリンク