Molecular Cloning of Two Caspase-Like Genes from the Hard Tick Haemaphysalis longicornis
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概要
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We identified two caspase-like genes from the midgut cDNA library of the hard tick, Haemaphysalis longicornis. Sequence analysis showed that these cDNAs encoded homologues of caspase-2 and caspase-8 that were categorized as apoptosis initiators. The H. longicornis caspase-2 (Hlcaspase-2) cDNA encodes 340 amino acid residues with a predicted molecular weight (Mw) of 38.5 kDa. Another cDNA identified as the H. longicornis caspase-8 (Hlcaspase-8) encodes 306 amino acid residues with an estimated Mw of 35.3 kDa. A catalytic active site was highly conserved in Hlcaspase-8 but not in Hlcaspase-2. RT-PCR analysis showed that both Hlcaspase-2 and Hlcaspase-8 were expressed in tick midgut and salivary glands. This is the first report of the molecular cloning of apoptosis-related genes in the tick.
- 公益社団法人 日本獣医学会の論文
著者
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Xuan Xuenan
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
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Fujisaki Kozo
National Institute Of Animal Health
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Tanaka Miho
National Research Center For Protozoan Diseases Obihiro University Of Agriculture And Veterinary Med
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NISHIKAWA Yoshifumi
National Research Center for Protozoan Deseases, Obihiro University of Agriculture and Veterinary Medicine
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LIAO Min
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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ZHOU Jinlin
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
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XUAN Xuenan
National Research Center for Protozoan Deseases, Obihiro University of Agriculture and Veterinary Medicine
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