ヒト消化管由来のCaco-2細胞によるポリフェノールの腸管吸収モデル実験

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Polyphenols distributed widely in vegetables, fruits, wine and teas are likely to have beneficial effects on human health, because of their antioxidative activities. Therefore we studied the efficiency of the polyphenol absorption in vitro using the human intestinal epithelial cell line, Caco-2. Caco-2 cells are being widely used as an in vitro model for human intestinal absorption potential. For the absorption experiments, Caco-2 cells were grown in 12-well culture plates (FALCON)at 37℃ under 5% CO_2-95% air atmosphere and constant humidity. The culture medium was changed every other day. After culturing for 2 weeks and removal of the medium, the cells were rinsed 3-4 times with phosphate buffer saline (PBS, pH7.4). PBS alone was added to the plate part, and polyphenol containing PBS to the insert part, and both parts were kept at room temperature for 1-4 hours. The ten kinds of polyphenols tested were D(+)-catechin, catechol, chlorogenic acid, quercetine, rutin, pyrogallol, EGCG, ECG, EC, and EGC. The polyphenols transferred from the insert to the plate were assayed by Folin-Dennis method. There were significant differences among various polyphenols in the efficiency of the intestinal absorption of polyphenols, when Caco-2 cells were employed. The quantity of polyphenol absorption was influenced by their concentrations in PBS. The absorption efficiency of chlorogenic acid was the highest among the 10 polyphenols tested. The ten kinds of polyphenols tested showed no corelation between their molecular weights and the quantity absorbed.

ヒト消化管由来のCaco-2細胞によるポリフェノールの腸管吸収モデル実験

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