Preparation of injectable 3D-formed β-tricalcium phosphate bead/alginate composite for bone tissue engineering
スポンサーリンク
概要
- 論文の詳細を見る
A novel, injectable bone tissue engineering material was developed that consisted of β-tricalcium phosphate (β-TCP) beads as the solid phase and alginate as the gel phase. To prepare the instantaneously formed composite scaffold, an aqueous calcium chloride solution was dried on the surface of β-TCP beads and crosslinked with an alginic acid sodium solution, thereby forming stable β-TCP beads and alginate gel which were injectable via a syringe. This biodegradable composite was a three-dimensional (3D) material that could be used as an injectable scaffold for bone tissue engineering. In particular, the composite with 2.0wt% alginate concentration exhibited a compressive strength of 69kPa in dry conditions, which was significantly higher than that exhibited by 1.0wt%. Furthermore, mesenchymal stem cells (MSC) were 3D-cultured within the composite and then investigated for osteogenic markers. MSC-loaded composite was subjected to scanning electron microscope (SEM) examination and implanted subcutaneously for in vivo experiment. Results showed that the scaffold provided support for osteogenic differentiation. In light of the encouraging results obtained, this novel injectable composite material may be useful for bone tissue engineering.
- 日本歯科理工学会の論文
著者
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tabata yasuhiko
Department of Biomaterials, Institute for Frontier Medical Science, Kyoto university
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MATSUNO Tomonori
Department of Oral and Maxillofacial Surgery, The Nippon Dental University, School of Life Dentistry
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OMATA Kazuhiko
Department of Oral and Maxillofacial Surgery, The Nippon Dental University, School of Life Dentistry
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HASHIMOTO Yoshiya
Department of Biomaterials, Osaka Dental University
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SATOH Tazuko
Department of Oral and Maxillofacial Surgery, The Nippon Dental University, School of Life Dentistry
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Satoh Tazuko
Department Of Oral And Maxillofacial Surgery School Of Life Dentistry At Tokyo The Nippon Dental Uni
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Satoh Tazuko
日本歯科大学 生命歯学部口腔外科
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Satoh Tazuko
Department Of Oral And Maxillofacial Surgery The Nippon Dental University School Of Life Dentistry A
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Satoh Tazuko
Department Of Oral & Maxillofacial Surgery The Nippon Dental University School Of Dentistry At T
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Hashimoto Yoshiya
日本歯科大学 生命歯学部口腔外科
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Hashimoto Yoshiya
Department Of Biomaterials Osaka Dental University
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Matsuno Tomonori
日本歯科大学 生命歯学部口腔外科
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Matsuno Tomonori
Department Of Oral And Maxillofacial Surgery School Of Life Dentistry At Tokyo The Nippon Dental Uni
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Matsuno Tomonori
Department Of Oral And Maxillofacial Surgery The Nippon Dental University School Of Life Dentistry A
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Matsuno Tomonori
Department Of Oral & Maxillofacial Surgery The Nippon Dental University School Of Dentistry At T
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Omata Kazuhiko
Department Of Oral And Maxillofacial Surgery School Of Life Dentistry At Tokyo The Nippon Dental Uni
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ADACHI Seita
Department of Biomaterials, Osaka Dental University
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YOSHITAKA Yamauchi
Department of Oral and Maxillofacial Surgery, School of Life Dentistry at Tokyo, The Nippon Dental U
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OZEKI Yasuyuki
New Material Science Laboratory, Advance Ltd.
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UMEZU Yoshikazu
New Material Science Laboratory, Advance Ltd.
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NAKAMURA Masaaki
Department of Biomaterials, Osaka Dental University
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Tabata Yasuhiko
Department Of Biomaterials Field Of Tissue Engineering Institute For Frontier Medical Sciences Kyoto
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Tabata Yasuhiko
Institute For Frontier Medical Sciences Kyoto University
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Tabata Yasuhiko
Department Of Oral And Maxillofacial Surgery The Nippon Dental University School Of Life Dentistry A
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Tabata Yasuhiko
Department Of Biomaterials Field Of Tissue Engineering Institute. For Frontier Medical Sciences Kyot
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Adachi Seita
Department Of Biomaterials Osaka Dental University
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Ozeki Yasuyuki
New Material Science Laboratory Advance Ltd.
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Tabata Yasuhiko
Department Of Biomaterials Kyoto University
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Umezu Yoshikazu
New Material Science Laboratory Advance Ltd.
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Yoshitaka Yamauchi
Department Of Oral And Maxillofacial Surgery School Of Life Dentistry At Tokyo The Nippon Dental Uni
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Hashimoto Y
Department Of Biomaterials Osaka Dental University
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Tabata Yasuhiko
Department Of Biomaterials Field Of Tissue Engineering Institute For Frontier Medical Sciences Kyoto
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Tabata Yasuhiko
Department Of Biomaterials Field Of Tissue Engineering Institute For Frontier Medical Sciences Kyoto
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Tabata Yasuhiko
Department Of Biomaterials Field Of Tissue Engineering Institute For Frontier Medical Sciences Kyoto
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Tabata Yasuhiko
Department Of Biomaterials Field Of Tissue Engineering Institute For Frontier Medical Sciences Kyoto
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Nakamura Masaaki
Department Of Biomaterials Osaka Dental University
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Yamaki Takahiko
Department Of Biomaterials Field Of Tissue Engineering Institute For Frontier Medical Sciences Kyoto
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Nakamura Masaaki
Department Of Applied Physics Faculty Of Science Tokyo University Of Science
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