Evidence that HSP70 Gene Expression May be Useful for Assessing the Cytocompatibility of Dental Biomaterials
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概要
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In the current studies, we examined the possibility of using HSP70 gene regulation as a cytocompatibility test for dental biomaterials. For this reason, we assessed the effects of three metal salts, HgCl_2, CuSO_4 and NiCl_2 on HSP70 gene expression in HeLa S3 cells using real-time Taqman quantitative PCR. Incubation of the cells for 4 h in medium containing HgCl_2 (20 or 40μM), CuSO_4 (157, 313, 625 or 1250μM) or NiCl_2 (5000 and 10000μM) significantly induced HSP70 mRNA. The real-time Taqman quantitative PCR was able to detect HSP70 mRNA induction at 4-fold lower concentrations of HgCl_2 and 8-fold lower concentrations of CuSO_4 than the Neutral Red cell viability assay. These results indicate that real-time Taqman quantitative PCR, in combination with the monitoring of cell viability, may be a valuable tool for distinguishing between specific HSP70 mRNA induction and cytocompatibility of metals in dental biomaterials.
- 日本歯科理工学会の論文
著者
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Ueda Akihiro
大阪歯科大学 歯科理工学講座
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Ueda Akihiro
Department Of Geriatric Dentistry Osaka Dental University
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Ueda Akihiro
Department Of Applied Chemistry And Biochemistry Faculty Of Engineering Kumamoto University
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HASHIMOTO Yoshiya
Department of Biomaterials, Osaka Dental University
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Hashimoto Yoshiya
Department Of Biomaterials Osaka Dental University
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NAKAMURA Masaaki
Department of Biomaterials, Osaka Dental University
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Nakamura Masaaki
Department Of Applied Physics Faculty Of Science Tokyo University Of Science
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UEDA Akihiro
Department of Biomaterials, Osaka Dental University
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