βTCP/Collagen Sponge Composite Enhances the Osteogenic Differentiation of Mesenchymal Stem Cells(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
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概要
- 論文の詳細を見る
For the bone regeneration, tissue engineering approach combines cells capable of osteogenic activity with an appropriate scaffold. We developed a biodegradable sponge composite (b-TCP/CS) by combining a-tricalcium phosphate (a-TCP) granules and collagen. Human mesenchymal stem cells (MSCs) were cultured within b-TCP/CS scaffolds and collagen sponge (CS) scaffolds, we investigated the alkaline phosphates (ALP) activity and the expression of osteogenic markers using RT-PCR. Furthermore, MSCs- loaded b-TCP/CS and CS were implanted under the back skin of nude mice for 4 and 12 weeks, and then removed for histological evaluation of bone formation. In vitro studies demonstrated that b-TCP/CS with MSCs of the ALP activity and the expression of osteogenic markers was higher than CS. In vivo, no bone formation was observed in CS, but newly bone formation was observed around a-TCP granules of b-TCP/CS. These results suggest that b-TCP/CS composites enhance the osteogenic differentiation of MSCs and new bone formation.
- 硬組織再生生物学会の論文
著者
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HASHIMOTO Yoshiya
Department of Biomaterials, Osaka Dental University
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SATOH Tazuko
Department of Oral and Maxillofacial Surgery, The Nippon Dental University, School of Life Dentistry
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Satoh Tazuko
Department Of Oral And Maxillofacial Surgery School Of Life Dentistry At Tokyo The Nippon Dental Uni
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Satoh Tazuko
日本歯科大学 生命歯学部口腔外科
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Satoh Tazuko
Department Of Oral And Maxillofacial Surgery The Nippon Dental University School Of Life Dentistry A
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Satoh Tazuko
Department Of Oral & Maxillofacial Surgery The Nippon Dental University School Of Dentistry At T
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Hashimoto Yoshiya
日本歯科大学 生命歯学部口腔外科
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Hashimoto Yoshiya
Department Of Biomaterials Osaka Dental University
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Matsuno Tomonori
日本歯科大学 生命歯学部口腔外科
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Matsuno Tomonori
Department Of Oral And Maxillofacial Surgery School Of Life Dentistry At Tokyo The Nippon Dental Uni
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Matsuno Tomonori
Department Of Oral And Maxillofacial Surgery The Nippon Dental University School Of Life Dentistry A
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Matsuno Tomonori
Department Of Oral & Maxillofacial Surgery The Nippon Dental University School Of Dentistry At T
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NAKAMURA Tatsuo
Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University
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KUREMOTO Koh-ichi
Department of Removable Prosthodontics and Occlusion, Osaka Dental University
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NAKAHARA Taka
Section of Developmental and Regenerative Dentistry, The Nippon Dental University School of Dentistr
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Kuremoto Ko-ichi
Department of Removable Prosthodontics and Occlusion, Osaka Dental University
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Nakahara Taka
Section Of Developmental And Regenerative Dentistry School Of Life Dentistry At Tokyo The Nippon Den
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Nakamura Tatsuo
Department Of Bioartificial Organs Institute For Frontier Medical Sciences Kyoto University
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Nakamura Tatsuo
Department Of Artificial Organs Institute For Frontier Medical Sciences Kyoto University
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Hashimoto Y
Department Of Biomaterials Osaka Dental University
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