97 Lupinus属植物中のルピン系アルカロイド生合成におけるアルカロイドアシル転移酵素の精製および諸性質(ポスター発表の部)

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In the cell-free extracts of 10-day-old seedlings of Lupinus termis F., the acyltransferase activities were detected for tigloyl-CoA: 13α-hydroxymultiflorine O-tigloyl transferase (HMTase) (EC.2.3.1.-) and tigloyl-CoA: 13α-hydroxylupanine O-tigloyltransferase (HLTase) (EC.2.3.1.-) (scheme 1). The HMTase has been purified approximately 6000-fold by seven steps involving different types of column chromatography. The HMTase is separated into two isoenzymes by chrmatofocusing on Mono P. A several alkaloid substrates and acyl-CoA derivatives were examined to clarify the substrate specificity of HMTase (Table 1). The HMTase can utilize only tetracyclic lupin alkaloid, which has 13 hydroxy group and same absolute configuration at 7 and 9 position such as (-)-13α-hydroxymultiflorine (4) and (+)-13α-hydroxylupanine (6), as substrate. On the other hand, the HMTase can utilize acetyl-, propionyl-, crotonyl-, and benzoyl-CoAs as acyl donor. Distribution of the acyltransferases activities in several lupin plant was investigated. The activities of the acyl-transferases were detected only in Lupinus and Cytisus species containing ester lupin alkaloids (Table 2). However, the plant in other genera, Thermopsis, Baptisia, Echinosophora, Naackia and Sopora, gave no acyltranferase activities.
天然有機化合物討論会の論文
1993-09-10