Purification and Properties of a Plant β-D-Glucuronidase from Scutellaria Root

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概要

• 論文の詳細を見る

• β-D-Glucuronidase (baicalinase, GUS [EC 3.2.1.31]) activity in the crude drug, Scutellaria root, was assayed in line with the quality control standards of Kampo (Japanese herbal) medicines. GUS was purified to homogeneity in the purification steps including DEAE-Sepharose Fast Flow and chromatofocusing used PBE^<TM>94 and Polybuffer 74. These results suggest that the Scutellaria GUS is composed of 55 kDa active subunits and that the isoelectric point of this enzyme is pH 5.4. Optimal catalytic activity was found at pH 4.7 in the pH range 3.6-6.2 in 50 mM Na-citrate buffer. The purified enzyme hydrolyzed baicalin and wogonin glucuronide, but did not hydrolyze glycyrrhizin or some β-glucosides found in other crude drugs. GUS activity in several crude drugs is also described.

• 公益社団法人日本薬学会の論文
• 1995-11-15

著者

• 村越 勇

  千葉大

• 山本 武司

  Central Research Laboratories Tsumura & Co.

• 池上 文雄

  Faculty Of Pharmaceutical Sciences Chiba University

• 池上 文雄

  Research Center of Medicinal Resources, Faculty of Pharmaceutical Sciences, Chiba University

• 松苗 薫

  Research Center of Medicinal Resources, Faculty of Pharmaceutical Sciences, Chiba University

• 久光 正文

  Research Center of Medicinal Resources, Faculty of Pharmaceutical Sciences, Chiba University

• 栗原 孝

  Central Research Laboratories, Tsumura & Co.

• 村越 勇

  Research Center of Medicinal Resources, Faculty of Pharmaceutical Sciences, Chiba University

• 松苗 薫

  Research Center Of Medicinal Resources Faculty Of Pharmaceutical Sciences Chiba University

• 久光 正文

  Research Center Of Medicinal Resources Faculty Of Pharmaceutical Sciences Chiba University

• 栗原 孝

  Central Research Laboratories Tsumura & Co.

• Ikegami Fumio

  Research Center Of Medicinal Resources Faculty Of Pharmaceutical Sciences Chiba University

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